Rapid detection of chromosome number aberration in spontaneously aborted fetuses using fluorescence in situ hybridization.
- Author:
Lan-lin SONG
1
;
Li XIONG
;
Si-ping LIU
;
Jie LIU
;
Mei ZHONG
;
Yun-fei GAO
;
Rui-feng WU
;
Yan-hong YU
Author Information
- Publication Type:Journal Article
- MeSH: Aborted Fetus; Chromosome Aberrations; Female; Humans; In Situ Hybridization, Fluorescence; methods; Karyotyping; Pregnancy
- From: Journal of Southern Medical University 2011;31(9):1605-1607
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess the value of fluorescence in situ hybridization (FISH) in the diagnosis of common chromosome number aberration in spontaneously aborted fetuses.
METHODA total of 100 spontaneously aborted fetuses were analyzed by G-banding and by FISH to test chromosome number aberration mainly for chromosomes 13, 18, 21, X and Y, and the results of FISH test was assessed according to those by G-banding test.
RESULTSFISH results were well consistent with those by G-banding test. FISH test identified trisomy in 32 samples and polyploidy in 7 samples. Two samples with cell culture failure were found to have trisomy 16 by FISH. Discrepancies in the results between the two tests occurred in 3 samples, but the results of FISH were verified by other methods. Kappa test between FISH technology and G-banding showed a good consistency between FISH and karyotyping (P<0.05).
CONCLUSIONFISH is an effective and rapid method for detecting chromosome number aberration in spontaneously aborted fetuses, and the combination of FISH and karyotyping provides more reliable diagnostic evidence.
