ANGPTL4 gene silencing by short-hairpin RNA inhibits the migration of human colorectal cancer cell line HT29.
- Author:
Jie HAN
1
;
Xiao-tong HU
;
Xue-feng HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Angiopoietin-like 4 Protein; Angiopoietins; genetics; Cell Movement; Colorectal Neoplasms; genetics; pathology; Gene Silencing; HT29 Cells; Humans; RNA, Small Interfering; genetics
- From: Journal of Southern Medical University 2011;31(9):1614-1618
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of ANGPTL4 gene silencing on the migration of human colon cancer cells in vitro.
METHODSThe expression of ANGPTL4 in human colorectal cancer cell lines was detected by semi-quantitative RT-PCR. Following stable transfection with a short-hairpin RNA (shRNA) targeting ANGPTL4 gene in HT29 cells, ANGPTL4 mRNA and protein expressions were detected by semi-quantitative RT-PCR and direct ELISA, respectively, and the changes in cell migration ability and cell morphology were observed with transwell and immunofluorescence assays.
RESULTSANGPTL4 was expressed in most of the colorectal cancer cell lines. Compared with the control groups, HT29 cells with shRNA-mediated ANGPTL4 gene silencing showed significantly decreased expression of ANGPTL4 mRNA and protein (P<0.05) and lowered cell migration ability possibly due to decreased pseudopodia formation.
CONCLUSIONANGPTL4 was expressed in most colorectal cancer cell lines. Decreased ANGPTL4 gene expression can inhibit the cell migration and pseudopodia formation in HT29 cells.
