Small interfering RNA targeting Apollon enhances the chemosensitivity of hepatocellular carcinoma cells in vitro.
- Author:
Jian-fa CHEN
1
;
Yu-hua LI
;
Yin-xiang CHEN
;
Kui-long XIE
;
Ming FU
;
Li LI
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Combined Chemotherapy Protocols; therapeutic use; Apoptosis; genetics; Carcinoma, Hepatocellular; pathology; Cell Line, Tumor; Hep G2 Cells; Humans; Inhibitor of Apoptosis Proteins; genetics; metabolism; Liver Neoplasms; pathology; RNA Interference; RNA, Small Interfering; genetics
- From: Journal of Southern Medical University 2011;31(10):1701-1704
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of small interfering RNA (siRNA) targeting Apollon in enhancing the chemosensitivity of hepatocellular carcinoma (HCC) cells in vitro.
METHODSHCC cells transfected with the siRNA targeting Apollon were tested for Apollon protein expression using Western blotting. MTT assay and ELISA were used to evaluate the proliferation and apoptosis of HCC cells transfected with siRNA after exposure to 5-FU or adriamycin.
RESULTSApollon siRNA obviously down-regulated Apollon protein expression in HCC cells. The siRNA-mediated suppression of Apollon expression resulted in a marked inhibition of cell growth and increased apoptotic rate of HCC cells, and enhanced both the growth-inhibiting and apoptosis-inducing effects of the chemotherapeutic drugs.
CONCLUSIONApollon siRNA can enhance the chemosensitivity of HCC cells to the chemotherapeutic drugs. Apollon can be a potentially important and feasible therapeutic target for HCC.
