Establishment of transgenic mice with visualized neovascularization.
- Author:
Zhen-lin LI
1
;
Yi GAO
;
Jun-shuang JIA
;
Song-qin CHEN
;
Hai-hong WANG
;
Jing AN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Base Sequence; Endothelium, Vascular; metabolism; Green Fluorescent Proteins; biosynthesis; genetics; Intermediate Filament Proteins; biosynthesis; genetics; Mice; Mice, Transgenic; Molecular Sequence Data; Neovascularization, Pathologic; genetics; Neovascularization, Physiologic; genetics; Nerve Tissue Proteins; biosynthesis; genetics; Nestin
- From: Journal of Southern Medical University 2011;31(10):1748-1752
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish transgenic mice with GFP expression in the vascular endothelium during neovascularization.
METHODSThe vector nestin-hsp68-gfp containing nestin second intron was introduced into U251 cells and the expression level of GFP was detected by fluorescence microscopy. Transgenic mice were produced by microinjection. The genome of the offspring mice was screened by PCR, and GFP expression in the vascular endothelium was detected using immunohistochemistry.
RESULTSThirteen offspring mice were obtained and 2 of them were positive for GFP in the vascular endothelium as detected by PCR. GFP was detected in the offspring mice both at the embryonic stage and after birth.
CONCLUSIONSThe transgenic mice with GFP expression in the vascular endothelium during neovascularization have been successfully established.
