Effect of CoCl2 pretreatment on Na + and K+ currents of the rat hippocampal neurons after acute hypoxia.
- Author:
Tong ZHAO
1
;
Wei LIU
;
Li-Ying WU
;
Ai-Shi DING
;
Fu-Zhuang WANG
;
Ming FAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Cell Hypoxia; Cobalt; pharmacology; Hippocampus; cytology; physiopathology; Neurons; drug effects; Patch-Clamp Techniques; Potassium Channels; metabolism; Rats; Rats, Wistar; Sodium Channels; metabolism
- From: Chinese Journal of Applied Physiology 2003;19(3):250-252
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study effect of CoCl2 pretreatment on the voltage-gated Na+ and K+ currents of the rat hippocampal neurons after acute hypoxia.
METHODSPrimarily cultured hippocampal neurons were divided into CoCl2 pretreated and non-pretreated groups. Patch clamp whole cell recording technique was used to examine Na+ and K+ currents of the hippocampal neurons.
RESULTSAfter acute hypoxia, I(Na) and I(K) of the hippocampal neurons were significantly decreased and the threshold of I(Na) was right-shifted. Pretreatment of the neurons with CoCl2 inhibited the reduction of I(Na) and I(K).
CONCLUSIONCcCl2 pretreatment alleviates the acute hypoxia-induced changes of I(Na) and I(K), which may be one of the mechanisms for the protective effect of CoCl2 on neurons.
