Experimental study on antagonism of tetrandrine in acute renal injury induced by gentamicin in guinea pig.
- Author:
Yu-Xiang AN
1
;
Hao TANG
Author Information
- Publication Type:Journal Article
- MeSH: Acetylglucosaminidase; urine; Actins; metabolism; Acute Kidney Injury; chemically induced; Animals; Benzylisoquinolines; pharmacology; Drug Antagonism; Drugs, Chinese Herbal; pharmacology; Gentamicins; adverse effects; Guinea Pigs; Transforming Growth Factor beta1; metabolism
- From: Chinese Journal of Applied Physiology 2003;19(3):278-281
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the antagonism of tetrandrine in acute renal injury induced by gentamicin in guinea pig.
METHODSThe experimental guinea pigs are divided into 4 groups by randomized blocks, they were group 1 (control), group 2 (tetrandrine), group 3 (gentamicin) and group 4 (gentamicin with tetrandrine). After 10 days, set apart urinary specimen for determination the activity of NAG, reserve renal specimen for observing the renal histology and measuring expression of actin and TGF-beta1.
RESULTSThe renal histology showed that the injury of group 3 was the most grievous, group 4 was lighter than group 3. In addition, the apoptosis was obviously less than group 3. The activity of urine of NAG in group 4 was lower than group 3. The levels of expression of actin and TGF-beta1 in group 4 were more than group 3.
CONCLUSIONWhen kidney is damaged by gentamicin, tetrandrine is able to reduce the activity of urinary NAG and inhibit the renal tubular epithelial cell apoptosis. Besides, tetrandrine can prevent breaking actin and evoke the endogenous transmission growth factor beta1 tune-up, the above results can indicate that tetrandrine has antagonism to acute renal injury induced by gentamicin in guinea pig.
