Glucocorticoids up-regulate human chorion 11beta-hydroxysteroid dehydrogenase type 1.
- Author:
Ping HE
1
;
Gang SUN
Author Information
- Publication Type:Journal Article
- MeSH: 11-beta-Hydroxysteroid Dehydrogenase Type 1; metabolism; Cells, Cultured; Chorion; drug effects; metabolism; Female; Glucocorticoids; pharmacology; Humans; RNA, Messenger; genetics; Up-Regulation; drug effects
- From: Chinese Journal of Applied Physiology 2003;19(3):291-294
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the co-localization of glucocorticoid receptor and 11beta-hydroxysteroid dehydrogenase 1 (11beta-HSD1) and to investigate whether glucocorticoids regulate the reductase activity and expression of 11beta-HSD1.
METHODSImmunohistochemical staining for 11beta-HSD1 in cultured primary human chorionic trophoblasts was performed. Radiometric conversion assay and Northern blot analysis were respectively used to observe the activity and mRNA expression of 11beta-HSD1.
RESULTS11beta-HSD1 and GR were co-expressed in the same chorionic trophoblast. Both 11beta-HSD1 reductase activity and mRNA levels were increased by dexamethasone (10(-6) mol/L, 10(-7) mol/L) in the cultured chorionic trophoblasts, and the effects were blocked by GR antagonist RU486 (10(-6) mol/L).
CONCLUSIONBy binding to GR, glucocorticoids induce the expression of 11beta-HSD1 by a possible intracrine mechanism.
