Effects of oxygen-glucose deprivation on cultured rat hippocampal neurons.
- Author:
Ai-Shi DING
1
;
Yah WU
;
Zi-Min MA
;
Hai-Sheng LI
;
Li-Ying WU
;
Ming FAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Hypoxia; Cells, Cultured; Glucose; deficiency; Hippocampus; cytology; Neurons; cytology; Oxygen; metabolism; Rats; Rats, Wistar
- From: Chinese Journal of Applied Physiology 2003;19(4):313-317
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effects of oxygen-glucose deprivation on cultured rat hippocampal neurons.
METHODSThe hippocampal neurons cultured for 12 d were exposed to combined oxygen-glucose deprivation for 0.5 - 4 h and then cultured with original medium in normoxia for 28 h. Necrotic neurons were identified by 0.4% trypan blue staining and apoptotic neurons were detected by a TUNEL technique. Meanwhile, the area, perimeter and circle diameter of cell bodies were measured respectively by a photography analysis system.
RESULTSThe percentage of necrotic cells in cultured hippocampal neurons increased significantly during oxygen-glucose deprivation, but the percentage of apoptotic cells increased significantly after 28 h oxygen-glucose recovery. Photography analysis showed that area, perimeter and circle diameter of the necrotic cell bodies were larger than those of the apoptotic ones.
CONCLUSIONOxygen-glucose deprivation can lead to severe damage of cultured hippocampal neurons. The necrosis is major during acute oxygen-glucose deprivation, while the apoptosis is major 28 h after oxygen-glucose recovery.
