UNLABELLEDTo study the effects of Bupivacaine and hyaluronidase on the proliferation of adult rat muscle satellite cells in vivo.

METHODSImmunohistochemistry, hematoxylin and eosin staining, electron micrograph were used.

RESULTS(1) There are few rare desmin positive satellite cells lie in the myofibers of control group and Sterile saline group which are still continual. MMD of control and Sterile saline group is 0.66% +/- 0.57% and 2.48% +/- 1.13% respectively. Sterile saline group has no significant difference than that of the control (P > 0.05). (2) The myofibers of hyaluronidase group are basically continual. The number of desmin positive satellite cells are increased. MMD of Hyaluronidase is 2.52% +/- 1.41% which has no remarkable difference than that of the Sterile saline (P > 0.05). (3) Plentiful necrosis and degeneration myofibers can been seen in Bupivacaine group and Hyaluronidase + Bupivacaine group coinciding with the activation and proliferation of muscle satellite cells. The number of Desmin positive satellite cells are increased significantly and some of which have formed myotubes. MMD of Bupivacaine and Hyaluronidase + Bupivacaine is 19.01% +/- 4.74% and 22.41% +/- 7.64% respectively which have significant change than that of Sterile saline (P < 0.01).

CONCLUSIONThe local anaesthetic Bupivacaine can induce the significant proliferation of myoblasts and the formation of myotubes in vivo. Hyaluronidase has no significant effect on the proliferation of satellite cells in vivo under this experimental condition.