The role of melatonin receptor and GABAA receptor in the sleeping time prolonged by melatonin in mice.
- Author:
Fang WANG
1
;
Dan ZOU
;
Jing-Cai LI
;
Chi HONG
;
Li-Bin CHEN
;
Xia CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bicuculline; pharmacology; Male; Melatonin; physiology; Mice; Mice, Inbred Strains; Picrotoxin; pharmacology; Prazosin; pharmacology; Receptors, GABA-A; physiology; Receptors, Melatonin; physiology; Sleep; physiology
- From: Chinese Journal of Applied Physiology 2003;19(4):402-405
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo observe the role of melatonin receptor and GABAA receptor in sleeping time prolonged by melatonin in mice.
METHODSThe absence of the righting reflex was considered as the sleep onset and the duration of the loss of the righting reflex was recorded as the sleeping time. The effects of receptor agonist and antagonist on hypnotic activity of melatonin were studied in the paper.
RESULTSPrazosin hydrochloride, the blocker of melatonin 3 receptor, didn't affect the sleeping time prolonged by melatonin in mice. GABA, the endogenous agonist of GABA receptor, significantly potentiated the hypnotic activity of melatonin. When picrotoxin, the ligand of picrotoxin site on GABAA receptor, used together with melatonin, it significantly antagonized the sleeping time prolonged by melatonin, however, bicuculline, the specific antagonist of GABA binding site in GABAA receptor, didn't affect the hypnotic activity of melatonin in mice.
CONCLUSIONMelatonin does not exhibit its potentiation sleeping time in mice through melatonin 3 receptor. Hypnotic activity of melatonin may be mediated through picrotoxin site on GABAA receptor.