The change of circulating endothelial cell in blood of rats and pulmonary small artery vascular endothelial cell under acute hypoxia.
- Author:
An-Liu WANG
1
;
Xiang-Yun WEN
;
Guo-Zhen LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Cell Count; Blood Circulation; Endothelial Cells; cytology; Endothelium, Vascular; cytology; Hypoxia; Lactate Dehydrogenases; metabolism; Pulmonary Artery; cytology; Rats; Rats, Wistar
- From: Chinese Journal of Applied Physiology 2004;20(1):15-18
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate changes of VEC and CEC under acute hypoxia.
METHODSObserve CEC in blood under acute hypoxia morphologically and count the number of CEC by optical microscope, measure LDH activity of young CEC and VEC by histochemical staining image analysis.
RESULTSLDH activities of VEC in hypoxic groups are lower than that in the group before hypoxia and decrease progressively with hypoxia time. LDH activities of young CEC in groups after hypoxia and before hypoxia are the same and are apparently lower than that of VEC. Before hypoxia most of CEC are aging, the number of CEC from hypoxic groups is greater than that before hypoxia and increases progressively with hypoxia time and most of CEC from hypoxic groups are young.
CONCLUSIONThe morphology and number of CEC may reflect the extent to which the vascular is injured. LDH activity of VEC may reflect the transformation from VEC into CEC. LDH activity of young CEC may reflect the extent to which the VEC is injured when falling from vascular wall.
