The effect of ginkgolide B on action potential, L-type calcium current and delayed rectifier potassium current in ischemic guinea pig ventricular myocytes.
- Author:
Xiao-Yan QI
1
;
Zhi-Xiong ZHANG
;
Qi-Qi CUI
;
Wei-Bin SHI
;
You-Qiu XU
Author Information
- Publication Type:Journal Article
- MeSH: Action Potentials; drug effects; Animals; Calcium Channels, L-Type; drug effects; Delayed Rectifier Potassium Channels; drug effects; Ginkgolides; pharmacology; Guinea Pigs; Heart Ventricles; drug effects; Lactones; pharmacology; Myocardial Ischemia; metabolism; Myocytes, Cardiac; drug effects; metabolism; Patch-Clamp Techniques
- From: Chinese Journal of Applied Physiology 2004;20(1):24-28
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effect of ginkgolide B from Ginkgo leave on action potential (AP), L-type calcium current (I(Ca) - L) and delayed rectifier potassium current (I(K)) in normal and ischemic guinea pig ventricular myocytes.
METHODSWith the standard microelectrode technique to record action potential and whole-cell variant patch-clamp technique to record calcium and potassium current.
RESULTS(1) Under normal condition, ginkgolide B shortened APD and had no effect on RP, AP and V(max). Ginkgolide B also increased I(K) in a concentration dependent manner and had no significant effect on I(Ca) - L (2) Under ischemia condition, it was observed that shortening of APD, APA, decrease V(max) and depolarization of RP was induced by ischemia, but ginkgolide B could attenuate above--mentioned changes. (3) Under ischemia condition, I(Ca) - L and I(K) were inhibited, perfusion with ischemia solution containing ginkgolide B could reverse the decrease of I(Ca) - L and I(K).
CONCLUSIONGinkgolide B had protective effect on ischemic myocardium to prevent ischemic arrhythmia.
