Effects of hsf1 genotype on the constitutive expression of alphaB-crystallin in mice myocardium.
- Author:
Li LIU
1
;
Hong-Hui ZHANG
;
Guo-Xian DING
;
Yun-Lin CHENG
;
Liang-Jun YAN
;
I J BENJAMIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; DNA-Binding Proteins; genetics; Female; Genotype; Heat Shock Transcription Factors; Male; Mice; Mice, Knockout; Myocardium; metabolism; Transcription Factors; genetics; alpha-Crystallin B Chain; genetics; metabolism
- From: Chinese Journal of Applied Physiology 2004;20(1):30-33
- CountryChina
- Language:Chinese
-
Abstract:
AIMTry to clarify the effects of HSF1 gene on the constitutively expressed alphaBC.
METHODSTo investigate the levels of constitutively expressed alphaB-Crystallin (alphaBC) in hsf1 knockout (hsf1 -/-) and hsf1 wild type (hsf1 +/+) mice myocardium by Western blot and immunohistochemistry.
RESULTSThe alphaBC levels in hsf1 -/- and hsf1 +/+ were 68.42% +/- 4.16%, 100% +/- 7.58%, respectively (P < 0.05, cytosolic fraction), and 20.53% +/- 1.01%, 37.55% +/- 1.91%, respectively (P < 0.05, pellet fraction). The alphaBC signals decreased significantly in hsf1 -/- myocardium compared with hsf1 +/+ myocardium stained with fluorescence immunohistochemistry.
CONCLUSIONhsf1 is the important, but not the only factor, which mediates the constitutively expressed alphaBC.
