Hypoxic preconditioning increase nPKCepsilon membrane translocation in the brain of mice.
- Author:
Xue-mei WANG
1
;
Jun-fa LI
;
Peng-yu ZU
;
Song HAN
;
Guo-wei LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blotting, Western; Brain; metabolism; Hippocampus; metabolism; Hypoxia; metabolism; Mice; Mice, Inbred BALB C; Protein Kinase C; metabolism; Protein Transport; physiology
- From: Chinese Journal of Applied Physiology 2004;20(2):105-109
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo explore the role of novel protein kinases C (nPKCs) in the development of cerebral hypoxic preconditioning.
METHODSBy using the mice model of hypoxic preconditioning, which was established before in our lab, the biochemistry techniques of SDS-PAGE and Western blot were applied to observe the effects of repetitive hypoxic exposure (H0-H4) on nPKCs (nPKCepsilon, delta, eta, mu and theta) membrane translocation in hippocampus and cortex.
RESULTSnPKCepsilon membrane translocation was increased in response to the hypoxic exposure times in the hippocampus (H0: 41.6% +/- 1.4% vs. H1-H4: 46.9% +/- 4.5%, 52.7% +/- 3.9%, 58.8% +/- 2.7% and 61.3% +/- 3.7%) and cortex (H0: 38.4% +/- 4.5% vs. 42.4% +/- 5.0%, 48.7% +/- 6.5%, 55.3% +/- 8.9% and 61.2% +/- 10.2%) of mice, and there were statistic significances among H2, H3 and H4 in hippocampus, and H3 and H4 in cortex respectively (P < 0.01). But for nPKCdelta, eta, mu and theta membrane translocation, there were no any significant changes in hippocampus and cortex of hypoxic preconditioned mice.
CONCLUSIONnPKCepsilon may play an important role in the development of cerebral hypoxic preconditioning, but it need more evidence to prove.
