Interleukin-2 reduces calcium handling capacity of rat ventricular myocytes during anoxia/reoxygenation.
- Author:
Chun-mei CAO
1
;
Qiang XIA
;
Zhi-guo YE
;
Yue-liang SHEN
;
Jun-zhu CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium; metabolism; Cell Hypoxia; Cells, Cultured; Heart Ventricles; cytology; Interleukin-2; pharmacology; Male; Myocardial Contraction; drug effects; Myocytes, Cardiac; drug effects; metabolism; Oxygen; metabolism; Rats; Rats, Sprague-Dawley; Sarcoplasmic Reticulum; metabolism
- From: Chinese Journal of Applied Physiology 2004;20(2):111-115
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effect of interleukin-2(IL-2) on the cell contractility and calcium handling in cardiomyocytes during normoxia or anoxia/reoxygenation.
METHODSChemical anoxia introduced by Krebs-Henseleit(K-H) solution containing 10(-3) mol/L sodium dithionite was used in the enzymatically isolated rat ventricular myocytes. The video-tracking system and spectrofluorometric method were employed to verify the cell contraction and calcium handling of the single myocyte.
RESULTSDuring anoxia, the cell contraction, amplitude of calcium transient induced by electrical stimulation and Ca2+ release induced by caffeine were depressed while resting calcium level was elevated, but the activity of the L-type calcium channels were not changed. All the parameters could not return to the pre-anoxia level during reoxygenation. IL-2 at 2 x 10(5) U/L administrated during anoxia aggravated the effect of reoxygenation on cell contraction and the calcium handling.
CONCLUSIONCoexistence of IL-2 during anoxia aggravated the effect of reoxygenation on the cell contraction and calcium handling in the isolated rat ventricular myocytes, in which the reduced calcium release from sarcoplasmic reticulum was involved.
