Lentivirus-mediated shRNA targeting ZNF217 suppresses cell growth, migration, and invasion of glioma cells in vitro.
- Author:
Qisheng LUO
1
;
Haineng HUANG
;
Yuanyang DENG
;
Huadong HUANG
;
Huangde FU
;
Kunxiang LUO
;
Chuanyu LI
;
Chengjian QIN
;
Zhanliang WEI
;
XueYu LI
Author Information
- Publication Type:Journal Article
- MeSH: Cadherins; metabolism; Cell Cycle; Cell Line, Tumor; Cell Movement; Cell Proliferation; Epithelial-Mesenchymal Transition; Genetic Vectors; Glioma; pathology; Humans; Lentivirus; Neoplasm Invasiveness; RNA, Messenger; RNA, Small Interfering; genetics; Trans-Activators; genetics; Transfection
- From: Journal of Southern Medical University 2015;35(7):1024-1033
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the role of ZNF217 in regulating cell proliferation, migration and invasion in glioma cells.
METHDOSA lentivirus-mediated shRNA-ZNF217 vector was infected into glioma U251 cells, and the interference efficiency was examined by Western blotting. MTT assay, flow cytometry, Transwell assay, and Boyden chamber assay were used to analyze the changes in cell proliferation, migration and invasion. Western blotting was used to detect the changes in ZNF217-related genes in the cells.
RESULTSshRNA-ZNF217 transfection significantly inhibited the expression of ZNF217 in U251 cells and suppressed the cell migration, invasion, growth, and cell cycle transition. ZNF217 knockdown downregulated the expression of pPI3, pAKT, C-Myc, and the mesenchyme biomarker N-cadherin, and stimulated the expression of the epithelium biomarker E-cadherin.
CONCLUSIONZNF217 promotes cell migration, invasion, and growth by activating PI3K/AKT signal to upregulate C-Myc and by modulating the genes associated with epithelial-mesenchymal transition in glioma cells.
