Anti-miR-145 promotes human airway smooth muscle cell proliferation and osteopontin synthesis in vitro.
- Author:
Peifen CHEN
1
;
Zhihui QIU
;
Guohua HUANG
;
Xiangmei ZHANG
;
Wujian PENG
;
Hui CENG
;
Wenyan LAI
Author Information
- Publication Type:Journal Article
- MeSH: Airway Remodeling; Apoptosis; Cell Proliferation; Cells, Cultured; Humans; MicroRNAs; antagonists & inhibitors; Myocytes, Smooth Muscle; drug effects; Osteopontin; biosynthesis; Respiratory System; cytology
- From: Journal of Southern Medical University 2015;35(7):1073-1075
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of anti-miR-145 on human airway smooth muscle cell (HASMC) proliferation and osteopontin systhesis in vitro and explore the mechanisms.
METHODSHASMCs were treated with 10-100 nmol/L anti-miR-145, and the cell proliferation and apoptosis were investigated using a CCK-8 assay and flow cytometry, respectively. The changes in osteopontin synthesis after the treatment was quantified with Western blotting.
RESULTSTreatment with 10 and 50 nmol/L anti-miR-145 significantly promoted the proliferation and osteopontin synthesis in HASMCs (P<0.05 or <0.01), and 50 nmol/L anti-miR-145 obviously inhibited the cell apoptosis (P<0.01).
CONCLUSIONAnti-miR-145 promotes HASMC proliferation and osteopontin synthesis and inhibits HASMC apoptosis in vitro, indicating the important role of anti-miR-145 in the pathogenesis of airway remodeling.
