Lactobacillus rhamnosus GG inhibits Cronobacter-induced meningitis in neonatal rats.
- Author:
Liang ZHONG
1
;
Ruqin LIN
;
Beiguo LONG
;
Xianbo WU
;
Hongying FAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Bacterial Adhesion; Caco-2 Cells; Cronobacter; pathogenicity; Enterobacteriaceae Infections; therapy; Humans; Intestines; microbiology; Lactobacillus rhamnosus; Meningitis, Bacterial; therapy; Probiotics; Rats
- From: Journal of Southern Medical University 2015;35(8):1079-1083
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the inhibitory effect of Lactobacillus rhamnosus GG ( LGG) against Cronobacter-induced meningitis in neonatal rats.
METHODSThe cell adhesion and invasion capacities of Cronobacter were assayed in Caco-2 cells, and the optimal time length and concentration of the bacterium for infection were determined. The suppressive effects of LGG on the adhesion and invasion of Cronobacter in caco-2 cells were tested by competitive and exclusion experiments, and its inhibitory effect against Cronobacter-induced meningitis was evaluated in neonatal rats.
RESULTSCronobacter showed aggressive adhesion to caco-2 cells with an optimal infection time of 3 h. LGG produced a concentration-dependent inhibition of Cronobacter adhesion and invasion by competing with and excluding the latter for cell adhesion. In neonatal rats, LGG showed an obvious preventive effect and also a moderate therapeutic effect against Cronobacter-induced meningitis.
CONCLUSIONLGG can inhibit Cronobacter entry across the intestinal barrier to achieve preventive and therapeutic effects against Cronobacter-induced meningitis.
