Minocycline reduces hyperpolarization-activated current in rat substantia gelatinosa neurons.
- Author:
Mengye ZHU
1
;
Nana LIU
;
Sicong PENG
;
Lingchao LI
;
Daying ZHANG
;
Tao LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Male; Minocycline; pharmacology; Neurons; drug effects; Patch-Clamp Techniques; Rats; Rats, Sprague-Dawley; Substantia Gelatinosa; cytology
- From: Journal of Southern Medical University 2015;35(8):1155-1161
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of minocycline on hyperpolarization-activated current (Ih) in the substantia gelatinosa (SG) neurons in rat spinal dorsal horn.
METHODSIn vitro spinal cord transverse slices were prepared from 3-5-week-old male Sprague-Dawley rats. Using whole-cell patch clamp technique, Ih currents were recorded before and after bath application of minocycline (1-300 µmol/L) to the SG neurons.
RESULTSIh currents were observed in nearly 50% of the recorded neurons, and were blocked by Ih blocker CsCl and ZD7288. Minocycline rapidly and reversibly reduced the amplitude of Ih and decreased the current density in a concentration-dependent manner with an IC50 of 34 µmol/L.
CONCLUSIONMinocycline suppresses the excitability of SG neurons through inhibiting the amplitude and current density of Ih and thereby contributes to pain modulation.
