Effects of anti-survivin oligonucleotides on growth of peritoneally implanted ovarian cancer xenografts in nude mice.
- Author:
Yan SUN
1
;
Jian-Min DI
;
Chun-Ning CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line, Tumor; Down-Regulation; Female; Humans; Inhibitor of Apoptosis Proteins; antagonists & inhibitors; metabolism; Interleukin-6; metabolism; Mice; Mice, Nude; Neoplasm Transplantation; Oligonucleotides; pharmacology; Ovarian Neoplasms; pathology; therapy; STAT3 Transcription Factor; metabolism
- From: Journal of Southern Medical University 2015;35(8):1211-1221
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of anti-survivin oligonucleotides (ASODN) on the invasion and growth of peritoneally implanted ovarian cancer cell xenografts in nude mice.
METHODSNude mouse models bearing peritoneally implanted ovarian cancer cell (SKOV3) xenografts were established and subjected to intraperitoneal injection of survivin ASODN or saline (control). The number and weight of the intraperitoneal xenografts were compared between the two groups.The expressions of interleukin (IL-6), signal transducer and activator of transcription3 (STAT3), phosphorylated STAT3 (p-STAT3), and survivin protein in the tumor tissues were detected with Western blotting in both groups.
RESULTSCompared with those in the control group, the number and weight of the intraperitoneal xenografts were significantly reduced in ASODN group (P<0.05). ASODN treatment also resulted in significantly lowered protein levels of IL-6, STAT3, p-STAT3, and survivin in the tumor tissues (P<0.05).
CONCLUSIONSurvivin ASODN can suppress the invasion and migration capacity of ovarian cancer cells and inhibit peritoneal metastasis of the tumor in nude mice possibly though down-regulation of IL-6/STAT3 signaling pathway.
