Nuclear factor I-C inhibits platelet-derived growth factor-induced enhancement of dermal fibroblast sensitivity to TGF-β.
- Author:
Liangping ZHANG
1
;
Yang WANG
;
Rui LEI
;
Hui SHEN
;
Yichen SHEN
;
Zhinan WU
;
Jinghong XU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Fibroblasts; drug effects; Genetic Vectors; Humans; Lentivirus; NFI Transcription Factors; genetics; Platelet-Derived Growth Factor; pharmacology; Protein-Serine-Threonine Kinases; metabolism; Proto-Oncogene Proteins c-sis; Receptors, Transforming Growth Factor beta; metabolism; Transfection; Transforming Growth Factor beta; pharmacology; Up-Regulation
- From: Journal of Southern Medical University 2015;35(9):1245-1250
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of nuclear factor I-C (NFI-C) on platelet-derived growth factor (PDGF)-induced up-regulation of TGF-β receptor II (TβRII) in dermal fibroblasts.
METHODSA lentiviral vector containing NFI-C sequence (Lenti-GFP-NFI-C) was transfected into a human foreskin fibroblast cell line (HFF-1). Cultured HFF-1 cells, cells transfected with Lenti-GFP-NFI-C, and cells transfected with a negative virus were stimulated with PDGF-BB, and Western blotting and RT-qPCR were used to detect the expression levels of TβRII in the treated cells.
RESULTSPDGF treatment significantly increased the expression level of TβRII in HFF-1 cells (P<0.05). The cells transfected with Lenti-GFP-NFI-C expressed a significantly lower level of TβRII than non-transfected cells in response to PDGF stimulation (P<0.05), but the negative virus showed no such inhibitory effect (P>0.05). No significant difference was found in the expression level of TβRII protein between cells transfected with Lenti-GFP-NFI-C-transfection before PDGF stimulation and the blank control cells.
CONCLUSIONNFI-C can inhibit PDGF-induced up-regulation of TβRII and thus reduce the sensitivity of the dermal fibroblasts to TGF-β.
