MicroRNA-133a antagonizes phenylephrine-induced hypertrophy of neonatal rat cardiomyocytes in vitro.
- Author:
Qi LI
1
;
Xiangsheng YANG
;
Xiaohua ZHOU
;
Lu XIAO
;
Xi LIN
;
Fengbo ZHANG
;
Lingli LI
;
Yanhong YU
;
Yanlin MA
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; Animals; Cells, Cultured; Genetic Vectors; Hypertrophy; MicroRNAs; genetics; Myocytes, Cardiac; cytology; pathology; Phenylephrine; adverse effects; RNA, Messenger; Rats; Transfection
- From: Journal of Southern Medical University 2015;35(9):1283-1286
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the mechanism of miR-133a in reversing neonatal rat cardiomyocyte hypertrophy induced by phenylephrine.
METHODSA miR-133a precursor cDNA was used to construct an adenovirus vector, which was transfected into 293 cells to harvest miR-133a-containing virus. Neonatal rat cardiac myocytes treated by phenylephrine were exposed to miR-133a adenovirus, and the changes in cell area was measured; the expression levels of miR-133a and Acta1, Actc1, Actb, Myh6, Myh7, and BNP mRNAs were detected by quantitative RT-PCR.
RESULTSPhenylephrine treatment increased the area of cardiomyocytes by more than 3 folds and significantly enhanced the expression levels of Acta1, Actc1, Actb, Myh6, Myh7 and BNP mRNAs. All these changes were obviously reverse by miR-133a treatment.
CONCLUSIONmiR-133a is an important regulator of phenylephrine-induced cardiomyocyte hypertrophy and negatively regulates this process.
