Protective effect of Apelin-13 on focal cerebral ischemia-reperfusion injury in rats.
- Author:
Guangyong WU
1
;
Liang LI
;
Daguang LIAO
;
Zhifei WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Brain Edema; Brain Ischemia; drug therapy; Intercellular Signaling Peptides and Proteins; pharmacology; Male; Malondialdehyde; metabolism; Oxidative Stress; Rats; Rats, Sprague-Dawley; Reperfusion Injury; drug therapy; Signal Transduction; Superoxide Dismutase; metabolism
- From: Journal of Southern Medical University 2015;35(9):1335-1339
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effect of Apelin-13 on focal cerebral ischemia-reperfusion injury in rats.
METHODSFocal transient cerebral ischemia-reperfusion injury was induced in male SD rats using modified suture occlusion technique. The rats were randomly divided into 5 groups: Sham group, Model group, Apelin-low dose (A) group, Apelin-middle dose (B) group and Apelin-high dose (C) group. Apelin-13 was injected into lateral cerebral ventricle, and the neurological function score, brain edema, infarct volume, apoptosis, malondialdehyde (MDA), superoxide dismutase (SOD) and extracellular regulated kinase1/2 (ERK1/2) protein were measured.
RESULTSNeurological function scores, percentage of brain water content, infarct volumes and TUNEL-positive cells in B and C groups were lower than those in Model group (P<0.05). The level of MDA in the tissue bomogenate of brain tissue in the surrounding area of ischemia of B and C groups was lower than that of Model group, while the activity of SOD was higher (P<0.05). There was no significant difference in ERK1/2 protein expression among the groups (P>0.05). P-ERK1/2 increased in Model group and A, B, and C groups compared with Sham group (P<0.05), and that of A, B, and C group was higher than that of Model group (P<0.05).
CONCLUSIONApelin-13 may play an important role by inhibiting oxidative stress to protect against focal cerebral ischemia-reperfusion injury; ERK1/2 signaling pathway may be involved in the protective mechanism of Apelin-13.