Protective effect of Sanhuangyinchi Fang drug serum on hydrogen peroxide-induced DNA oxidative damage in LO2 cells.
- Author:
Huan DAI
1
;
Jian-Xin DIAO
;
Jin-Ying OU
;
Hai-Ye LI
;
Yun-Gao YANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Comet Assay; DNA Damage; Deoxyguanosine; analogs & derivatives; Drugs, Chinese Herbal; pharmacology; Humans; Hydrogen Peroxide; toxicity; Oxidation-Reduction; Oxidative Stress; Protective Agents; pharmacology; Reactive Oxygen Species
- From: Journal of Southern Medical University 2015;35(10):1434-1439
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the protective effect of Sanhuangyinchi Fang drug serum (SF) against hydrogen peroxide-mediated DNA oxidative damage in LO2 cells.
METHODSThe LO2 cells were randomly divided into the control group, H(2)O(2) group, SF groups (5%, 10%, and 15%) and vitE group. The morphological features of the treated LO2 cells were observed under inverted microscope. The viability of the treated cells was assessed with CCK-8 method, and the activity of SOD, CAT and GSH-PX were detected biochemically. Reactive oxygen species (ROS) levels, the content of 8-OHdG, and DNA damage of the cells were evaluated by flow cytometry, ELISA, and Comet assay, respectively.
RESULTSCompared with H(2)O(2) group, the cells in SF groups (10% and 15%) and vitE group showed higher cell survival rate (P<0.05) and higher SOD, CAT, GSH-PX (P<0.05) and ROS scavenging activities (P<0.01) with markedly decreases the content of 8-OHdG (P<0.01) and reduced tailing ratio, tail length, tail moment and Olive tail moment (P<0.05).
CONCLUSIONSF drug serum, especially at the concentration of 15%, can protect LO2 cells from H(2)O(2)-mediated DNA oxidative damage.
