Danshen Modulates Nrf2-mediated Signaling Pathway in Cisplatin-induced Renal Injury
10.1007/s11596-017-1801-1
- Author:
CAO SI-SI
1
;
YAN MIAO
;
HOU ZHEN-YAN
;
CHEN YING
;
JIANG YUN-SHENG
;
FAN XIN-RONG
;
FANG PING-FEI
;
ZHANG BI-KUI
Author Information
1. Department of Pharmacy
- Keywords:
cisplatin;
nephrotoxicity;
Danshen;
Nrf2;
oxidative stress
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2017;37(5):761-765
- CountryChina
- Language:Chinese
-
Abstract:
Danshen,an efficacious agent for cardiovascular diseases,has been found to play an essential role in kidney injury.In the present study,the effect of Danshen on cisplatin-induced renal dysfunction was investigated in a mouse model.Danshen was administered to mice at a dose of 3 g/kg 4 days before and 3 days after cisplatin treatment.A single intraperitoneal injection of 20 mg/kg cisplatin was used to induce nephrotoxicity.The mice were sacrificed 72 h after cisplatin intoxication.Biochemical parameters including serum creatinine and blood urea nitrogen were analyzed.Histopathological changes of kidney tissues were detected using HE staining.Antioxidant enzymes (GSH-Px and SOD) and peroxidative product (MDA) were detected.Protein expressions of Nrf2 and its target genes including HO-1 and NQO1 were measured by Western blotting.The results showed that pretreatment with Danshen significantly reduced serum creatinine and blood urea nitrogen in the cisplatin-treated mice.Histopathological examination showed that Danshen mitigated the renal damage induced by cisplatin.Moreover,Danshen restored the activities of antioxidant enzymes (GSH-Px and SOD) and normalized the MDA contents in renal tissues.Western blotting revealed that Danshen enhanced the expressions of Nrf2 and its target genes in cisplatin-exposed mice.It was suggested that Danshen protects against the cisplatin-induced renal impairment in the mice,which is potentially associated with the upregulation of Nrf2-mediated signaling pathway.
