Detection of Low-abundance Point Mutations by Competitive Strand Assisted Endonuclease Ⅳ Signal Amplification System
10.1007/s11596-017-1808-7
- Author:
XIONG FEI
1
;
LIU CHUAN-ZHEN
;
LI WANG-QIANG
;
DONG ZI-QIANG
;
ZHAN JIE
Author Information
1. Department of Urology
- Keywords:
low-abundance point mutation;
competitive DNA probe;
endonuclease Ⅳ;
melting temperature discrimination
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2017;37(5):803-806
- CountryChina
- Language:Chinese
-
Abstract:
Genetic mutations are important molecular biomarkers for cancer diagnosis and surveillance.Therefore,the development of methods for mutation detection characterized with straightforward,highly specific and sensitive to low-level mutations within various sequence contexts is extremely needed.Although some of the currently available methods have shown very encouraging results,their discrimination efficiency is still very low.Herein,we demonstrate a fluorescent probe coupled with blocker and property of melting temperature discrimination,which is able to identify the presence of known or unknown single-base variations at abundances down to 0.1％ within 20 min.The discrimination factors between the perfect-match target and single-base mismatched target are determined to be 10.15-38.48.The method is sequence independent,which assures a wide range of application.The new method would be an ideal choice for high-throughput in vitro diagnosis and precise clinical treatment.
