Construction of antisense human tankyrase-1 RNA retroviral vector and its inhibition on tongue cancer cells.
- Author:
Yu-xin JI
1
;
Ping ZHANG
;
Wei-min CHEN
;
Sheng-rong ZHU
;
Xue-jin TAO
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Line, Tumor; Cell Proliferation; Genetic Therapy; Genetic Vectors; genetics; Humans; RNA, Antisense; genetics; Retroviridae; genetics; Tankyrases; genetics; Telomerase; metabolism; Tongue Neoplasms; genetics; metabolism; pathology; therapy; Transfection
- From: Chinese Journal of Stomatology 2007;42(3):180-183
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the inhibition of telomerase activity and cellular proliferation in tongue cancer TCCA-8113 cell lines by antisense human tankyrase-1 RNA treatment, and explore the possibility of the tankyrase-1 as a target of gene therapy for tongue cancer.
METHODSThe replication deficient retrovirus expressing tankyrase-1 antisense RNA was constructed to infect the TCCA-8113 cells. Tankyrase-1 expression was examined by RT-PCR. Telomerase activity was assayed by telomerase repeat amplification protocol (TRAP). Cell proliferation was investigated by cellular growth curve. Cellular apoptosis was detected by flow cytometry method and invert microscope.
RESULTSTankyrase-1 expression and telomerase activity of tongue cancer TCCA-8113 cells were significantly inhibited. There was G(1)-S phase arrest when TCCA-8113 cells were treated with antisense tankyrase-1 transduction. Cellular proliferation was arrested, and cellular apoptosis occurred after antisense tankyrase transduction.
CONCLUSIONSThe transduction of antisense tankyrase-1 by retroviral vector can significantly inhibit the tankyrase-1 expression and telomerase activity of tongue cancer TCCA-8113 cell lines, and arrest the cellular proliferation and promote cellular apoptosis. The tankyrase may be a potential target of gene therapy for tongue cancer.
