Inhibition of proliferation of salivary adenoid cystic carcinoma by targeting gene transfer.
- Author:
Tao SU
1
;
Hong-chen SUN
;
Guang-xiang ZANG
;
Hong ZHANG
;
Dong-min LU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Carcinoma, Adenoid Cystic; genetics; pathology; Cell Line, Tumor; Cell Proliferation; Gene Targeting; Genetic Vectors; Humans; Salivary Gland Neoplasms; genetics; pathology; TNF-Related Apoptosis-Inducing Ligand; genetics; Telomerase; genetics; Transfection
- From: Chinese Journal of Stomatology 2007;42(3):184-185
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of gene TRAIL driven by human telomerase reverse transcriptase (hTERT) promoter in SACC-83 cell tumor necrosis factor related apoptosis in dncing ligand.
METHODSAfter pACTERT-TRAIL plasmid transfected was into SACC-83 and HEL cells through liposome, the expression of TRAIL was examined using RT-PCR technique, the cells' survival rate by methyl thiazolyl tetrazolium (MTT) method and apoptosis rate by flow cytometry.
RESULTSExpression of extrinsic TRAIL gene driven by hTERT promoter was detected in SACC-83 cells, and not detected in human embryonic lung fibroblast (HEL) cells. After transfection of pACTERT-TRAIL, the proliferation of SACC-83 cells was significantly inhibited, and its apoptotic rate was promoted, whereas no inhibited effect was observed on HEL cells and its apoptotic rate showed little change.
CONCLUSIONShTERT promoter can be used to induce tumor-specific expression of TRAIL gene and apoptosis in SACC-83 cells.
