DNA methylation of human telomerase reverse transcriptase promoter region in human ameloblastoma.
- Author:
Ming ZHONG
1
;
Ying YAN
;
Jie WANG
;
Jie LIU
;
Le LI
;
Yan-Bing GONG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Ameloblastoma; genetics; DNA Methylation; Female; Gene Expression Regulation, Neoplastic; Humans; Jaw Neoplasms; genetics; Male; Middle Aged; Promoter Regions, Genetic; genetics; Telomerase; genetics; Young Adult
- From: Chinese Journal of Stomatology 2007;42(5):304-307
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the DNA methylation of human telomerase reverse transcriptase (hTERT) promoter in ameloblastoma (AB) and investigate its clinical biological significance.
METHODSDNA methylation of hTERT promoter in 12 cases of AB and 11 cases of normal oral mucosa were detected by methylation-specific PCR.
RESULTSThe positive cases of unmethylated hTERT promoter region in AB and normal oral mucosa were 4 (4/12) and 6 (6/11), respectively (P < 0.001). The positive cases of methylated hTERT promoter region in AB and normal oral mucosa were 11 (11/12) and 3 (3/11), respectively. Four cases of AB and 1 case of normal oral mucosa showed both DNA methylation and unmethylation of hTERT promoter region in the same time.
CONCLUSIONSDNA methylation of of hTERT promoter region in AB was more common than that in normal oral mucosa. The DNA methylation in hTERT promoter maybe regulate hTERT gene expression.
