Effect of arecoline on the differentiation of myofibroblasts of oral mucosa.
- Author:
Xia LI
1
;
Tian-You LING
;
Yi-Jun GAO
Author Information
- Publication Type:Journal Article
- MeSH: Actins; metabolism; Arecoline; pharmacology; Cell Differentiation; drug effects; Cells, Cultured; Coculture Techniques; Fibroblasts; cytology; metabolism; Humans; Keratinocytes; cytology; Mouth Mucosa; cytology; Oral Submucous Fibrosis; metabolism; pathology
- From: Chinese Journal of Stomatology 2007;42(7):423-427
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the origin of myofibroblasts in oral submucous fibrosis.
METHODSThe oral keratinocytes and fibroblasts were isolated and cultured. The expression of the alpha-smooth muscle actin in the fibroblasts was examined by immunohistochemistry and reverse transcriptase polymerase chain reaction (RT-PCR).
RESULTSNo difference was found in expression of alpha-smooth muscle actin between the fibroblasts that were directly stimulated by arecoline and the control. The expression of alpha-smooth muscle actin in the keratinocyte and fibroblast-cocultured group was higher than in the control group, and higher in fibroblasts cocultured with keratinocytes preprocessed by arecoline than in fibroblasts cocultured with keratinocytes without preprocessed by arecoline.
CONCLUSIONSThe differentiation of myofibroblasts from fibroblasts in oral submucous fibrosis might be induced by the interaction of arecoline and keratinocyte.
