Localization and expression of CLIC1 in hepatocarcinoma ascites cell lines with high or low potentials of lymphatic spread.
- Author:
Mei-ying SONG
1
;
Jian-wu TANG
;
Ming-zhong SUN
;
Shu-qing LIU
;
Bo WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Ascites; metabolism; pathology; Blotting, Western; Cell Line, Tumor; Cell Membrane; metabolism; Chloride Channels; metabolism; Cytoplasm; metabolism; Immunohistochemistry; Liver Neoplasms, Experimental; metabolism; pathology; Lymphatic Metastasis; Mice; Mice, Inbred Strains; Two-Dimensional Difference Gel Electrophoresis
- From: Chinese Journal of Pathology 2010;39(7):463-466
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the localization and expression of CLIC1 in mouse hepatocarcinoma ascites cell lines with different metastatic potentials.
METHODSMouse hepatocarcinoma ascites models (a high potential of lymphatic metastasis cell line-Hca-F, and a low potential of lymphatic metastasis cell line-Hca-P) were investigated using fluorescent two-dimensional difference-gel electrophoresis (2-D DIGE) and mass spectrometry for detecting the localization and expression of CLIC1. Immunofluorescence, immunocytochemistry and Western blot were used to assess CLIC1 protein status in the two cell lines.
RESULTSCLIC1 expression was obtained in the cytoplasm and plasma membrane of cells in both cell lines. 2-D DIGE showed that CLIC1 was overexpressed in Hca-F cells, 1.6 folds higher than that of the Hca-P cells. Hca-F cells also had a higher integral membrane CLIC1 in the Hca-P cells.
CONCLUSIONSAlthough CLIC1 expression is detected in both Hca-F and Hca-P cell lines, a higher protein expression level is present in Hca-F cells. CLIC1 may play an important role in tumor metastasis.
