Detection of chromosomal DNA imbalance in medulloblastoma by comparative genomic hybridization.
- Author:
Ya-jing SUN
1
;
Shi-zhu YU
;
Cui-yun SUN
;
Qian WANG
;
Shu-mei JIN
;
Wei-xiang WU
;
Tong-ling AN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Age Factors; Cerebellar Neoplasms; genetics; Child; Child, Preschool; Chromosome Aberrations; Chromosome Deletion; Comparative Genomic Hybridization; DNA, Neoplasm; genetics; Female; Humans; Male; Medulloblastoma; genetics; Sex Factors; Young Adult
- From: Chinese Journal of Pathology 2010;39(9):606-610
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between chromosomal genomic DNA imbalance in medulloblastoma (MB), and the age and gender.
METHODSThe gains and losses of chromosomal genomic DNA in 16 MBs were analyzed using comparative genomic hybridization.
RESULTSThe gains and(or) losses were found in 15 of the 16 cases. There was not significant difference (P > 0.05) between the total gains (10/16) and losses (11/16). Both of their differences had also no significance between different age and gender groups (P > 0.05). In 15 cases with gains and(or) losses, single-, two-, three- and multi-chromosome genomic DNA imbalances were 3/15, 4/15, 1/15 and 7/15 respectively. Eleven gain zones (+5q, +6q, +7q, +11q, +15q, +17p, +17q, +19q, +20q, +21q, +Xp) and twenty-five loss zones (-1p, -1q, -2p, -2q, -3q, -4p, -6p, -6q, -8p, -8q, -10p, -10q, -11p, -14q, -16p, -16q, -17p, -18p, -18q, -19p, -19q, -20p, -20q, -Xp, -Xq) were detected in those tumors. +7q (6/16), +17q (6/16), -14q (5/16) and -10q (3/16) were the most frequent, but -14q only occurred in the cases of > 10-year-old.
CONCLUSIONSMost MBs have chromosomal genomic DNA imbalances. The frequent imbalance zones are mainly at the long arms of some chromosomes. +7q, +17q, -14q and -10q correlate closely to development of the tumors. -14q is important factor to result in MBs of > 10-year-old group. MB has possibly different molecular genetics subtype.
