Diverse components of voltage-gated calcium channel currents in primary cultured hippocampal neurons.
- Author:
Zhi-Ying LIN
1
;
Xiao-Chun CHEN
;
Jing ZHANG
;
Tian-Wen HUANG
Author Information
1. Department of Neurology, the Affiliated Union Hospital of Fujian Medical University, Fuzhou 350001, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Calcium;
physiology;
Calcium Channels;
physiology;
Cells, Cultured;
Hippocampus;
cytology;
Neurons;
physiology;
Patch-Clamp Techniques;
Primary Cell Culture
- From:
Acta Physiologica Sinica
2012;64(6):687-694
- CountryChina
- Language:Chinese
-
Abstract:
The present study was to record and separate the voltage-gated calcium channel currents (VGCCs) in primary cultured hippocampal neurons. An improved method is described for efficient and stable recording of VGCCs from primary cultured hippocampal neurons. The procedure allows the obtained hippocampal neurons overcome the defects from acutely isolated hippocampal neurons and currents do not rundown apparently after 20 min of membrane rupture. Using whole-cell configurations of patch clamp technique, it is shown that the method does not damage the neuron membrane electrical properties, and successfully records a variety of VGCCs components in hippocampal neurons (such as L, N, P/Q, T, etc.).
