Forskolin inhibits spontaneous contraction of gastric antral smooth muscle in rats.
- Author:
Jing-Zhi JIANG
1
;
Qian SUN
;
Dong-Yuan XU
;
Mo-Han ZHANG
;
Li-Hua PIAO
;
Ying-Lan CAI
;
Zheng JIN
Author Information
1. Department of Histology and Embryology, Medical College of Yanbian University, Yanji 133002, China.
- Publication Type:Journal Article
- MeSH:
Adenylyl Cyclases;
metabolism;
Animals;
Colforsin;
pharmacology;
Cyclic AMP;
pharmacology;
Cyclic AMP-Dependent Protein Kinases;
metabolism;
Isoquinolines;
pharmacology;
Muscle, Smooth;
drug effects;
Pyloric Antrum;
drug effects;
Rats;
Rats, Wistar;
Sulfonamides;
pharmacology
- From:
Acta Physiologica Sinica
2013;65(2):224-228
- CountryChina
- Language:Chinese
-
Abstract:
The aim of the present study was to investigate the effects of cyclic adenosine monophosphate (cAMP) on rat gastric antral circular smooth muscle function. Forskolin, a direct activator of adenylyl cyclase (AC), was used to observe the influences of cAMP. Multi-channel physiological recorder was used to record spontaneous contraction activity of gastric antral circular muscle from Wistar rats. And ELISA method was used to detect the change of cAMP production in perfusate. The results showed that forskolin concentration-dependently suppressed the amplitude and frequency of the spontaneous contraction of the gastric antral muscle, and lowered the baseline of contraction movement significantly. Forskolin concentration-dependently increased the production of cAMP in the perfusate, which showed a significant negative correlation with the contraction amplitude of gastric antral ring muscle. The inhibitory effect of forskolin on spontaneous contraction activity of rat gastric antral circular muscle could be blocked by cAMP-dependent protein kinase (PKA) inhibitor H-89. These results suggest forskolin increases cAMP production and then activates PKA pathway, resulting in the inhibition of the spontaneous contraction activity of rat gastric antral circular smooth muscle.
