Gene chip analysis of mutation of HBV-DNA open reading frame.
- Author:
Shi-jun CHEN
1
;
Wen-jun DU
;
Yan-qin LU
;
Wei XU
;
Yong AN
Author Information
- Publication Type:Journal Article
- MeSH: DNA, Viral; genetics; Hepatitis B Core Antigens; blood; Hepatitis B Surface Antigens; blood; Hepatitis B virus; genetics; Hepatitis B, Chronic; genetics; immunology; Humans; Mutation; Oligonucleotide Array Sequence Analysis; methods; Open Reading Frames; genetics
- From: Chinese Journal of Experimental and Clinical Virology 2004;18(4):373-376
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the mutation of HBV-DNA open reading frame (ORF), provide evidences for clinical diagnosis and treatment.
METHODSBy using gene chip technique, HBV-DNA was amplified by PCR, incorporated with fluorescence, hybridized with oligonucleotide to detect the gene sequence of DNA by computer analysis and observe the natural mutation of HBV-DNA ORF.
RESULTSThe mutations of HBV-DNA ORF existed widespread. The rates of mutation on Pre-C 1896, PreC 1814, BCP 1762, BCP 1764, P 528, P 552MI, P 552MV were 23.5%, 3.9%, 55.9%, 53.9%,39.2%, 38.2%, 10.8%, respectively.
CONCLUSIONThe gene chip technique possesses extremely high sensitivity and reliability,it is one of the effective methods to detect gene mutation. The mutation of HBV-DNA has important influence on the stability and progress of the disease, and on the judgement of prognosis.