Influence of the mutants of hepatitis B surface antigen on the cell immunity.
- Author:
Yu BAI
1
;
Guo-liang XIA
;
Yu GUO
;
Yu CONG
;
Zhi-yuan JIA
;
Min-zhuo GUO
;
Hong-lan ZHAO
;
Mei-yun ZHAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; CHO Cells; Cell Proliferation; drug effects; Cells, Cultured; Cricetinae; Cricetulus; Enzyme-Linked Immunosorbent Assay; Hepatitis B Surface Antigens; genetics; Humans; Interferon-gamma; metabolism; Interleukin-10; metabolism; Interleukin-2; metabolism; Lymphocytes; cytology; drug effects; metabolism; Mutant Proteins; genetics; Mutation; Plasmids; genetics; Recombinant Proteins; pharmacology; Transfection
- From: Chinese Journal of Experimental and Clinical Virology 2005;19(2):124-127
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the influence of the mutants of hepatitis B surface antigen on the cell immunity.
METHODSThe recombinant plasmids of NS2Swt, NS2S126, NS2S133, NS2S141 and NS2S145 were transfected into Chinese hamster ovary (CHO) cells and the expressed proteins were detected by means of ELISA. Following PHA-activated lymphoblasts proliferation assay and IFN-gamma, IL-2, IL-10 induction assay were done with these proteins.
RESULTSIt was identified that these proteins of HBsAg could stimulate human lymphoblasts proliferation. Besides, there were no significant difference between the mutants and the wild. It was deserved to point out that the HBsAg with T126S mutation could increase the expression of IFN-gamma in the culture medium while the HBsAg with M133T mutation induced more expression of IL-10.
CONCLUSIONThe results suggested that the cellular immune response to mutants of HBV might not be strengthened or weakened. But it should not be ignored that HBV T126S or M133T mutation may assert a potential impact on the cell immunity.
