Screening for new binding proteins which interact with BM2 of influenza B virus with yeast two-hybrid system.
- Author:
Hong YU
1
;
Li-hong YAO
;
Ai-jun CHEN
;
Jie HE
;
Run-qing JIA
;
Cong-sheng CHENG
;
Zhi-qing ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Angiopoietin-like Proteins; Angiopoietins; genetics; metabolism; DNA-Binding Proteins; genetics; metabolism; Gene Library; Humans; Influenza B virus; genetics; metabolism; Kidney; metabolism; Oxo-Acid-Lyases; genetics; metabolism; Plasmids; genetics; Protein Binding; Protein-Arginine N-Methyltransferases; genetics; metabolism; Repressor Proteins; genetics; metabolism; Ribosomal Proteins; genetics; metabolism; Transcription Factors; genetics; metabolism; Two-Hybrid System Techniques; Viral Proteins; genetics; metabolism; Zinc Fingers; genetics
- From: Chinese Journal of Experimental and Clinical Virology 2005;19(2):182-184
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the role of BM2 protein in the life cycle of influenza B virus.
METHODSThe authors screened human kidney MATCHMAKER cDNA library for new binding partners of BM2 of influenza B virus by using the yeast two hybrid system with truncated BM2 (26-109 aa) as the bait.
RESULTSSix positive plasmids encoding N-acetylneuraminate pyruvate lyase, angiopoietin 3, zinc finger protein 251, ribosomal protein S20, protein arginine N-methyltransferase 1 variant 1 (PRMT) and transcription factor-like 1 (TCFL1) were obtained.
CONCLUSIONThe results suggest that BM2 may play an important role in the life cycle of influenza B virus.
