Molecular characteristics of three new isolates of Japanese encephalitis virus in Fujian Province.
- Author:
Duan CHEN
1
;
Huan-yu WANG
;
Shi-hong FU
;
Hong SONG
;
Juan DENG
;
Yi-liang YANG
;
Guo-dong LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Animals; China; DNA, Complementary; chemistry; genetics; Encephalitis Virus, Japanese; classification; genetics; isolation & purification; Encephalitis, Japanese; virology; Genome, Viral; Genotype; Humans; Mice; Molecular Sequence Data; Phylogeny; RNA, Viral; genetics; isolation & purification; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Viral Envelope Proteins; genetics
- From: Chinese Journal of Experimental and Clinical Virology 2005;19(1):5-8
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the genotype of newly isolated Japanese encephalitis viruses in Fujian province and the characteristics of amino acid sequence in the E gene.
METHODSPrM and E segments of newly isolated Japanese encephalitis viruses were amplified by RT-PCR, the PCR products were cloned into T vector for sequencing. Phylogenetic analysis was carried out by PHYLIP program.
RESULTSNewly isolated Japanese encephalitis viruses belonged to genotype III, the nucleotide and amino acid of E gene showed high homology to vaccine strain SA14-14-2, but had some difference in some domains.
CONCLUSIONNewly isolated viruses in Fujian province belonged to Japanese encephalitis virus genotype III. E protein of the isolates showed some differences as compared with vaccine strains.
