Construction of infectious Japanese encephalitis virus clone based on the cDNA template of the attenuated live vaccine production strain SA14-14-2.
- Author:
Ming ZENG
1
;
Li-li JIA
;
Yong-xin YU
;
Guan-mu DONG
;
Wen-xue LIU
;
Zhi-wei WANG
;
De-fu LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Base Sequence; Cells, Cultured; Cercopithecus aethiops; Cloning, Molecular; DNA, Complementary; genetics; Encephalitis Virus, Japanese; genetics; immunology; pathogenicity; Encephalitis, Japanese; pathology; virology; Genome, Viral; Japanese Encephalitis Vaccines; immunology; Mice; RNA, Viral; genetics; Vaccines, Attenuated; immunology; Vero Cells; Virulence
- From: Chinese Journal of Experimental and Clinical Virology 2005;19(1):9-11
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct infectious Japanese encephalitis virus (JEV) based on the in vitro-ligated cDNA template of the vaccine strain SA14-14-2, and identify the virus.
METHODSFull-length genomic cDNA of JEV SA14-14-2 strain was ligated and then RNA was transcribed in vitro, the infective virus was obtained by transfecting the RNA into Vero cells and identified.
RESULTSThe infective clone of JEV was constructed, the virulence was weaker than the wild virus.
CONCLUSIONIt was possible to construct infectious clone from the production strain of live attenuated Japanese B encephalitis vaccine.
