Analysis of part of M gene and genotyping for M segment of hantavirus detected from HFRS patients' sera in Qingdao region during 2000 - 2003.
- Author:
Gang QIAO
1
;
Shu-shen PANG
;
Hong-le ZHANG
;
Yan GONG
;
Mao-ling CHENG
;
Zhen-hai LI
Author Information
- Publication Type:Journal Article
- MeSH: China; Genotype; Hantavirus; genetics; Hemorrhagic Fever with Renal Syndrome; blood; virology; Humans; RNA, Viral; blood; genetics; isolation & purification; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA; Viral Matrix Proteins; genetics
- From: Chinese Journal of Experimental and Clinical Virology 2005;19(1):22-24
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the molecular epidemiological characteristics of hantavirus seen during 2000-2003 in Qingdao region of Shandong province.
METHODSSera were collected from 64 patients with hemorrhagic fever with renal syndrome (HFRS) and viral RNA was extracted from the sera. HTN and SEO universal primers were designed as outer primers and HTN and SEO specific primers as inner primers. G1 gene region of M segment from hantavirus was amplified by using RT-nest-PCR for sequencing. The data of nucleotide sequences were analyzed by DNA star software.
RESULTSSix cases were positive by HTN specific primer of total cases (9%); 25 of 64 cases by SEO specific primer (39%); total positive rate was 48%. In general, SEO type was a prevalent type of hantavirus in Qingdao region. The variation of the nucleotide sequences among SEO viruses (nucleotide sequence divergence ranged from 0.3% approximately 8.9%) was lower than that among HTN type (nucleotide sequence divergence ranged from 2.6% approximately 11.2% ).
CONCLUSIONMajority of hantavirus found in Qingdao region belonged to SEO type and still a few strains belonged to HTN type. Most of the HTN viruses were detected in Jiaonan county.
