Expression of HBV preS2/S gene in mammalian cells transferred with adenoviral vector.
- Author:
Chun-liang LEI
1
;
Cheng-hui HUANG
;
Zhan YANG
;
Xiao-ping TANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Animals; Cell Line; Cell Line, Tumor; Cercopithecus aethiops; Enzyme-Linked Immunosorbent Assay; Gene Expression; Genetic Vectors; genetics; Green Fluorescent Proteins; genetics; metabolism; Hepatitis B Surface Antigens; genetics; metabolism; Hepatitis B virus; genetics; immunology; Humans; Microscopy, Fluorescence; Reverse Transcriptase Polymerase Chain Reaction; Transfection; Vero Cells
- From: Chinese Journal of Experimental and Clinical Virology 2005;19(1):55-57
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study HBV preS2/S gene expression effects in mammalian cells transferred with recombinant adenoviral vector.
METHODSThe replication-deficient recombinant adenoviral vector (Ad-HBs) carrying HBV preS2/S gene were constructed by homologous recombination in bacteria. The 293 cells, Vero cells, HepG2 cells and mesenchymal stem cells (MSCs) were infected with adenoviruses. The expressions of enhanced green fluorescent protein (EGFP) were observed with fluorescence microscope and the expressions of HBsAg were detected by RT-PCR and ELISA in vitro.
RESULTSMore than 90% of 293 cells, Vero cells, HepG2 cells or MSCs expressed EGFP after transfection at the MOI of 20 and the titers of HBsAg were more than 3.229 (A value) in culture supernatant.
CONCLUSIONThe HBV preS2/S gene was not only expressed efficiently in immortalized cells, but also expressed efficiently in stem cells with the recombinant adenoviruses vector.
