Identification of oligopeptides mimicking the virus attachment protein of hantaan virus.
- Author:
Xin LÜ
1
;
Xiao-ping XUE
;
Qiao-xin YANG
;
Wen YIN
;
Ying-feng LEI
;
Fang-lin ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; CHO Cells; Cell Membrane; metabolism; Cercopithecus aethiops; Cricetinae; Cricetulus; Enzyme-Linked Immunosorbent Assay; Epitopes; immunology; metabolism; Hantaan virus; immunology; Microscopy, Confocal; Oligopeptides; immunology; metabolism; Peptide Library; Vero Cells; Viral Proteins; immunology; metabolism
- From: Chinese Journal of Experimental and Clinical Virology 2005;19(1):58-60
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify and characterize the epitope associated with the virus attachment protein (VAP) of hantaan virus.
METHODSThe monoclonal antibody 3G1 was used as the ligand to biospan from a phage-displayed 12-amino acid peptide library, then the positive phage clones were chosen and sequenced. The amino acid sequences of them were compared with that of hantaan virus G2 in homology. The characteristics of positive phage were studied by IFA and ELISA. A decapeptide combining to cell membrane was observed under laser scanning confocal microscope (LSCM).
RESULTSThe conservative motif PX(1-2) HX(0-2) H displaying on positive clones shared homologous amino acid sequence with G2 96YPWHTAKCHY105.
CONCLUSIONG2 96YPWHTAKCHY105 might play some roles in virus binding to host cell, and might be a possible key epitope of hantaan virus VAP.
