Design and rapid evaluation of a TaqMan assay for the detection of influenza A viruses.
- Author:
Ji-ming CHEN
1
;
Zhi-liang WANG
;
Zeng-chang PANG
;
Cheng-ying SUN
;
Ying-xue SUN
;
Cui-ping SONG
;
Ying YI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Birds; Humans; Influenza A virus; genetics; isolation & purification; Influenza in Birds; diagnosis; virology; Influenza, Human; diagnosis; virology; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; methods; Sensitivity and Specificity
- From: Chinese Journal of Experimental and Clinical Virology 2005;19(1):80-83
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo design and rapidly evaluate a TaqMan assay for detecting influenza A viruses.
METHODSThe probe and the primers of the assay were designed with the software packages of DNA Star and Primer Premier 5.0. Their specificity and conservation were verified through Blast in GenBank and electronic hybridization. The assay's sensitivity was compared with the standard RT-PCR.
RESULTSThe designed primers and probe were confirmed to be very specific and conserved. The assay was 3-27 folds more sensitive than the standard RT-PCR. The RT and PCR steps could be simplified into one step.
CONCLUSIONThe TaqMan Real-time PCR assay is specific, sensitive and easy to perform.
