Cloning, expression and purification of interferon-kappa, a novel human interferon, and its antiviral activity.
- Author:
Hui ZHANG
1
;
Zhao-jun DUAN
;
Jian-gao ZHU
;
Fu-wang PENG
;
Wu-ping LI
;
Han-chun GAO
;
Zhi-ping XIE
;
Yu-e WANG
;
Yun-de HOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antiviral Agents; pharmacology; Cell Line; Cell Proliferation; drug effects; Cercopithecus aethiops; Cloning, Molecular; DNA, Complementary; genetics; Electrophoresis, Polyacrylamide Gel; Escherichia coli; genetics; Gene Expression; Humans; Interferon Type I; genetics; isolation & purification; pharmacology; K562 Cells; Killer Cells, Natural; cytology; drug effects; immunology; Microbial Sensitivity Tests; Plasmids; genetics; Recombinant Proteins; isolation & purification; metabolism; pharmacology; Vero Cells
- From: Chinese Journal of Experimental and Clinical Virology 2005;19(3):223-226
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare human interferon-k (hIFN-kappa) and study its biological activities.
METHODSWhole length of hIFN-kappa's cDNA was cloned, and its sequence was chemically synthesized according to the optimized codons of E.coli, then was expressed in E.coli DH5alpha. After purified, the rhIFN-kappa protein was tested for its various kinds of biological activities.
RESULTSThe purity of rhIFN-kappa was above 90%. In WHIS-VSV system, the antiviral activity of rhIFN-kappa was 2.0 x 10(6) IU/mg. Compared with rhIFN-alpha-2b, the biological activities of rhIFN-kappa were all feeble, including antiviral activity, promoting NK cell activity and anti-proliferation activity.
CONCLUSIONAntiviral activities of rhIFN-kappa on cell lines of different species are different, different viruses show different sensitivity to rhIFN-kappa.
