Cytokines secretion by peripheral blood mononuclear cells from hepatitis C patients after stimulation with synthetic peptides at the highly variable region.
- Author:
Shu-ping CHI
1
;
Jing LI
;
Rong GAO
;
Hai-bin SU
;
Bo-an LI
;
Wei LI
;
Yun CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Cells, Cultured; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Hepatitis C; blood; virology; Humans; Interferon-gamma; metabolism; Interleukin-10; metabolism; Interleukin-4; metabolism; Leukocytes, Mononuclear; cytology; drug effects; metabolism; Oligopeptides; chemical synthesis; chemistry; pharmacology; Time Factors
- From: Chinese Journal of Experimental and Clinical Virology 2005;19(3):279-281
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETest the cytokines secretion by peripheral blood mononuclear cells (PBMC) from hepatitis C patients after stimulation with highly variable region (HVR1) synthetic peptides.
METHODSELISA was used to test the cytokines secretion, flow cytometry to group the proliferated cells, and the antigenicity of synthetic peptide was predicted by the computer modeling.
RESULTS(1)There were PBMC proliferation when stimulated by HVR1 antigen synthetic peptides. (2) There was a rise of IFN-gamma, IL-4 and IL-10. But no rise of IL-2 and TNF-gamma was found. (3) The proliferated cells were mainly CD4+ lymphocytes.
CONCLUSIONPMBC from hepatitis C patients tend to secret Th2 cytokines after stimulation with HVR1 designed by the authors.
