Molecular biological identification of Batai virus isolated in China.
- Author:
Shi-hong FU
1
;
Xiao-hong SUN
;
Huan-yu WANG
;
Yu-xi CAO
;
Huan-qin WANG
;
Wei-bin LIU
;
San-ju TAO
;
Guo-dong LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Animals; Bunyamwera virus; classification; genetics; isolation & purification; China; Culicidae; virology; DNA, Complementary; chemistry; genetics; Molecular Sequence Data; Phylogeny; RNA, Viral; genetics; isolation & purification; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA; Sequence Homology, Amino Acid
- From: Chinese Journal of Experimental and Clinical Virology 2005;19(4):331-334
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo study the molecular characteristics of YN92-4 strain isolated from mosquitoes in Yunnan Province and define its classification.
METHODSThe S segment of YN92-4 strain was amplified and sequenced by 2 different sets of primers. The phylogenic tree of S fragment was constructed by Phylip bio-software. The amino acid sequences of N and NSs proteins were also studied.
RESULTSYN92-4 strain could be amplified by 2 sets of primers respectively, S segment showed a highest homology with Batai virus (X73464), reached 96.4%, the homology of protein N and NSs amio-acid sequence with Batai virus was 99.1% and 98% respectively.
CONCLUSIONThe YN92-4 strain belongs to Batai virus, this is the first report of molecular biological identification of Batai virus in China.
