Sequential analyses of circulating HBV specific T helper cell response in chronic hepatitis B patients receiving antiviral treatment.
- Author:
Min WANG
1
;
Ling-xia ZHANG
;
Sheng-qiang LUO
;
Dong-ping XU
;
Chuan-lin ZHU
;
Zi-rong TANG
;
Fu-sheng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Antiviral Agents; therapeutic use; DNA, Viral; blood; genetics; Enzyme-Linked Immunosorbent Assay; methods; Female; Hepatitis B Core Antigens; immunology; Hepatitis B virus; drug effects; genetics; immunology; Hepatitis B, Chronic; blood; drug therapy; virology; Humans; Lamivudine; therapeutic use; Male; T-Lymphocytes, Helper-Inducer; cytology; drug effects; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2005;19(4):387-390
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo investigate the frequency of circulating HBV specific T helper cell and evaluate its association with serum levels of HBV DNA before and during lamivudine treatment in patients with chronic hepatitis B.
METHODSThe frequency of circulating HBV specific T helper cells in response to HBcAg in 25 chronic HBV-infected patients was determined by Elispot assay; serum HBV DNA was quantitated by real-time PCR.
RESULTSThe frequency of HBV specific T helper cell before antiviral treatment (47.30 +/- 25.50 SFCs /1 x 10(6) PBMC) was significantly higher than that at the third month of therapy (23.10 +/- 18.45 SFCs /1 x 10(6) PBMC, P < 0.05). All 8 patients observed dynamically had decreased frequency of HBV specific T helper cell at the third month of therapy; six patients with serum HBV DNA level reduced had higher frequency of HBV specific T helper cell before treatment than 2 patients without serum HBV DNA level decrease.
CONCLUSIONHBV specific T helper cell response at the time of hepatitis flare in chronic hepatitis B patients was significantly augmented compared to that at the time of catabasis.
