Dynamic expression of hepatitis B virus covalently closed circular DNA in 2.2.15 cell.
- Author:
Mao-chang LIU
1
;
Gui-qiang WANG
;
Wen-hua PIAO
;
Hong-li XI
;
Hai-ying LU
;
Yan WANG
;
Qin-huan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; DNA, Circular; genetics; DNA, Viral; chemistry; genetics; Hepatitis B Surface Antigens; analysis; Hepatitis B e Antigens; analysis; Hepatitis B virus; genetics; immunology; Humans; Polymerase Chain Reaction; methods; Sequence Analysis, DNA
- From: Chinese Journal of Experimental and Clinical Virology 2005;19(4):391-394
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo determine the presence of covalently closed circular DNA (cccDNA), and to investigate the expression kinetics of HBV DNA, HBsAg and HBeAg in 2.2.15 cell.
METHODSHBV cccDNA was assessed by polymerase chain reaction, HBV DNA was measured by Taqman quantitative PCR and HBsAg and HBeAg was measured by EIA.
RESULTSHBV cccDNA was found in both intracellular and extracellular space. There was a good correlation between HBsAg, HBeAg and HBV DNA in the supernatant of 2.2.15 cell (r= 0.833, P < 0.05 and r= 0.939, P < 0.01 for HBsAg and HBeAg, respectively), whereas there was no significant correlation between intracellular HBV DNA levels and virus antigen levels (r= 0.024, P= 0.955 and r= 0.177; P= 0.625 for HBsAg and HBeAg, respectively).
CONCLUSIONHBV cccDNA was detectable in the culture medium and intracellularly in 2.2.15 cells, and these data provided an indication of HBV replication in 2.2.15 cell.
