Protective effect of hemoglobin-induced heme oxygenase-1 on injured lungs caused by limb ischemia-reperfusion in rats.
- Author:
Junlin ZHOU
1
;
Xiaoguang ZHU
;
Guisheng ZHANG
;
Tong LING
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blotting, Northern; Blotting, Western; Carboxyhemoglobin; analysis; Disease Models, Animal; Heme Oxygenase (Decyclizing); analysis; biosynthesis; Heme Oxygenase-1; Hemoglobins; pharmacology; Lung Diseases; etiology; pathology; Male; Probability; RNA, Messenger; biosynthesis; Rats; Rats, Sprague-Dawley; Reference Values; Reperfusion Injury; complications; metabolism; Sensitivity and Specificity
- From: Chinese Journal of Traumatology 2002;5(2):86-91
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo determine the role of hemoglobin (HB) induced heme oxygenase-1 (HO-1) in injured lungs caused by limb ischemia-reperfusion (I/R) in rats.
METHODSA rat model of ischemia in the hind limbs was made by clamping the infrarenal aorta with a microvascular clip, and lung injury occurred after reperfusion. To induce the expression of HO-1 in the lungs, Hb was administrated intraperitoneally at 16 hours before reperfusion. Northern blotting and Western blotting were used to detect the expression of HO-1 in the lungs, and the carboxyhemoglobin (COHb) level in arterial blood was assayed. The effect of hemoglobin (Hb) on the injured lungs after limb I/R was determined by measuring the changes of lung histology, polymorphonuclear (PMN) count, malondialdehyde (MDA) content and wet-to-dry weight ratio (W/D). Zinc protoporphyrin (ZnPP), an inhibitor of HO, was used to determine whether HO-1 was induced by Hb after lung injury.
RESULTSHb led to a significant increase in HO-1 mRNA and protein expression in the lungs, accompanied by the increase of COHb level in arterial blood. Compared with the sham controls, the lung PMN count, MDA content and W/D significantly increased at 4 hours after limb I/R, which reversed by the pretreatment with Hb at 16 hours before reperfusion. ZnPP blocked this protective role of Hb in the injured lungs.
CONCLUSIONSHb can induce the lung HO-1 expression, which plays an important role in the defense against I/R induced lung injury in rats.
