Sequential changes of hypoxia-inducible factor 1 alpha in experimental spinal cord injury and its significance.
- Author:
Yan JU
1
;
Min HE
;
Boyong MAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Biomarkers; analysis; DNA-Binding Proteins; analysis; metabolism; Disease Models, Animal; Female; Flow Cytometry; Hypoxia-Inducible Factor 1; Hypoxia-Inducible Factor 1, alpha Subunit; Injury Severity Score; Ischemia; pathology; Nuclear Proteins; analysis; metabolism; Probability; Proto-Oncogene Proteins c-bcl-2; metabolism; Rats; Rats, Sprague-Dawley; Spinal Cord Injuries; metabolism; pathology; Transcription Factors
- From: Chinese Journal of Traumatology 2002;5(2):103-106
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the sequential changes of HIF-1alpha (hypoxia-inducible factor 1 alpha) in experimental spinal cord injury in rats and to analyze its potential effects in SCI.
METHODSA static compression model of SCI was employed in this study. Expressions of HIF-1alpha were measured with immunohistochemical staining, while flow cytometry was used to determine the apoptotic ratio and bcl-2 expressions.
RESULTSHIF-1alpha began to increase 1 day after injury, and reached the peak at 3-7 days. Two weeks later, it declined significantly. The sequential changes of HIF-1alpha coincided well with the alterations of apoptotic ratio and contents of bcl-2.
CONCLUSIONSHIF-1alpha possibly participates in the secondary ischemic and hypoxic procedures after spinal cord injury, and may mediate the traumatic apoptosis. Further understanding of HIF-1alpha may provide new therapeutic regimens for SCI.
